Other
Part:BBa_M31999:Design
Designed by: Iny Jhun Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07 (2007-02-27)
Refactoring between HpaI and BamHI sites
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
All the overlaps between genes are removed, repeat sequences are remapped, and cut sites are added for easier manipulation of the sequence.
Source
It comes from the genomic sequence of M13K07.