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Part:BBa_M31999:Design

Designed by: Iny Jhun   Group: BE.109 Instructors BE.109 2006 MIT 20.109 Spring07 MIT 20.109 Fall07   (2007-02-27)


Refactoring between HpaI and BamHI sites


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 721
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

All the overlaps between genes are removed, repeat sequences are remapped, and cut sites are added for easier manipulation of the sequence.


Source

It comes from the genomic sequence of M13K07.

References